Spinocerebellar Ataxia Type 3 antibody | knockout validation | Millipore MAB5360

This is a knockout-validated antibody summary, based on the publication "Ataxin-3 promotes genome integrity by stabilizing Chk1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Spinocerebellar Ataxia Type 3 antibody | knockout validation | Millipore MAB5360 figure 1
Figure 1. Lysates of WT and ATX3-KO MEF cells were immunoblotted with the indicated antibodies. From [1].
Antibody information

Mouse monoclonal IgG1

Company: Millipore

Antibody: Spinocerebellar Ataxia Type 3

Catalog number: MAB5360

Summary: Mouse monoclonal IgG1 against human ataxin-3 fragment from aa F112-L249 as a fusion protein. Reacts with human, mouse, rat and monkey. Suitable for western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry and ELISA.

Validation Method

Western blot

Sample

WT and ATX3-KO MEF cells.

Primary incubation

1:10,000 dilution.

Secondary incubation

Peroxidase-conjugated secondary antibodies.

Detection

Chemiluminescence (Millipore).

References
  1. Tu Y, Liu H, Zhu X, Shen H, Ma X, Wang F, et al. Ataxin-3 promotes genome integrity by stabilizing Chk1.. Nucleic Acids Res. 2017;45:4532-4549 pubmed publisher