Spi-B antibody | knockout validation | Cell Signaling 14337

This is a knockout-validated antibody summary, based on the publication "Polycomb Repressive Complex 2 Regulates Genes Necessary for Intestinal Microfold Cell (M Cell) Development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Spi-B antibody | knockout validation | Cell Signaling 14337 figure 1
Figure 1. Spi-B protein expression in Spi-B KO and Scrambled cells generated by lentiCRISPR v2 genome editing in C57BL/6JRj intestinal organoids. Organoid lysates were analyzed by Western blot. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Spi-B

Catalog number: 14337

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the carboxy terminus of human Spi-B protein. Reacts with human and mouse. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and Spi-B KO C57BL/6JRj mouse intestinal organoids.

Primary incubation

At 4°C overnight

Secondary incubation

1:5,000 dilution horseradish-peroxidase–conjugated anti-rabbit (cat no:RABHRP1-10UL; Sigma-Aldrich) for 1 hour at room temperature.

Detection

Signal was detected using ECL reagent (cat no: 2232; Amersham, Amersham, UK).

References
  1. George J, Oittinen M, Martin Diaz L, Zapilko V, Iqbal S, Rintakangas T, et al. Polycomb Repressive Complex 2 Regulates Genes Necessary for Intestinal Microfold Cell (M Cell) Development. Cell Mol Gastroenterol Hepatol. 2021;12:873-889 pubmed publisher