Sp3 antibody | knockout validation | Santa Cruz sc-365220

This is a knockout-validated antibody summary, based on the publication "Robust hematopoietic specification requires the ubiquitous Sp1 and Sp3 transcription factors", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western Blot of nuclear extracts from E14 WT, Sp1ΔDBD/ΔDBD and Sp3-/- ESC showing protein expression of full length Sp1, Sp3 and histone H3 as a loading control. From [1].

Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: Sp3

Catalog number: sc-365220

Summary: Mouse monoclonal IgG1 (kappa light chain) specific for an epitope mapping between amino acids 627-651 at the C-terminus of human Sp3. Recommended for detection of Sp3 of mouse, rat, human and avian origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including canine, bovine, and porcine.

Validation Method

Western blot

Sample

WT and Sp3-/- ESC.

Blocking agent

4% milk powder in 0.1% TBS-Tween.

References

Gilmour J, O Connor L, Middleton C, Keane P, Gillemans N, Cazier J, et al. Robust hematopoietic specification requires the ubiquitous Sp1 and Sp3 transcription factors. Epigenetics Chromatin. 2019;12:33 pubmed publisher

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