Sox2 antibody | knockout validation

Sox2 antibody | knockout validation | Cell Signaling 23064

This is a knockout-validated antibody summary, based on the publication "HIF1α/HIF2α-Sox2/Klf4 promotes the malignant progression of glioblastoma via the EGFR-PI3K/AKT signaling pathway with positive feedback under hypoxia", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of the indicated proteins in control and Sox2-KO GBM cells. From [1].

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Sox2

Catalog number: 23064

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Ala188 of human Sox2 protein. Reacts with human and mouse. Suitable for western blot, immunoprecipitation, immunofluorescence (frozen), immunocytochemistry, flow cytometry (fixed/permeabilized) and Chromatin IP.

Validation Method

Western blot

Sample

Control and Sox2-KO glioblastoma (GBM) primary cells.

Primary incubation

1:1,000 dilution.

References

Wang P, Zhao L, Gong S, Xiong S, Wang J, Zou D, et al. HIF1α/HIF2α-Sox2/Klf4 promotes the malignant progression of glioblastoma via the EGFR-PI3K/AKT signalling pathway with positive feedback under hypoxia. Cell Death Dis. 2021;12:312 pubmed publisher

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