SorLA/SORL1 antibody | knockout validation | Abcam ab190684

This is a knockout-validated antibody summary, based on the publication "SORL1 deficiency in human excitatory neurons causes APP-dependent defects in the endolysosome-autophagy network", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

SorLA/SORL1 antibody | knockout validation | Abcam ab190684 figure 1
Figure 1. Total SORL1 levels are reduced by approximately 50% in SORL1 heterozygous KO neurons and completely absent in SORL1 homozygous KO neurons compared with isogenic control as detected by western blot analysis. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: SorLA/SORL1

Catalog number: ab190684

Summary: Rabbit monoclonal antibody against a recombinant fragment of SorLA/SORL1. Reacts with rat and human. It is predicted to react with mouse. Suitable for western blot and immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

Control and SORL1-KO human iPSCs

Detection

Li-Cor Odyssey system.

References
  1. Hung C, Tuck E, Stubbs V, van der Lee S, Aalfs C, van Spaendonk R, et al. SORL1 deficiency in human excitatory neurons causes APP-dependent defects in the endolysosome-autophagy network. Cell Rep. 2021;35:109259 pubmed publisher