Somatostatin Receptor 5 antibody | knockout validation | Abcam ab156864

This is a knockout-validated antibody summary, based on the publication "Characterization of agonist-dependent somatostatin receptor subtype 2 trafficking in neuroendocrine cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Somatostatin Receptor 5 antibody | knockout validation | Abcam ab156864 figure 1
Figure 1. Immunoblot showing the disappearance of SSTR5-specific bands in the two SSTR5-KO cell lines. A nonspecific band detected by the antibody is indicated. Please see Figure 1c in the article [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: Somatostatin Receptor 5

Catalog number: ab156864

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to SSTR5. Reacts with human and it is predicted to work with mouse and rat. Suitable for western blot, and flow cytometry. Unsuitable for immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) or immunoprecipitation.

Validation Method

Western blot

Sample

WT and SSTR5-KO AtT20 cell lines

Blocking agent

5% milk/TTBS buffer.

Primary incubation

1/5,000 dilution overnight at 4 °C.

Secondary incubation

Conjugated with horseradish peroxidase.

Detection

Chemiluminescence.

References
  1. Alshafie W, Pan Y, Kreienkamp H, Stroh T. Characterization of agonist-dependent somatostatin receptor subtype 2 trafficking in neuroendocrine cells. Endocrine. 2020;: pubmed publisher