This is a knockout-validated antibody summary, based on the publication "CRISPR/Cas9n-Mediated Deletion of the Snail 1Gene (SNAI1) Reveals Its Role in Regulating Cell Morphology, Cell-Cell Interactions, and Gene Expression in Ovarian Cancer (RMG-1) Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of Snail1 in wild-type (WT) RMG1 cells, Snail1 KO1 cells, and Snail1 KO1 cells transiently transfected with a Snail1 expression vector (Snail1 KO1 + Snail1 cells). Vinculin was used as a loading control. From [1].

Mouse monoclonal IgG1

Company: Cell Signaling

Antibody: Snail1

Catalog number: 3895

Summary: Mouse monoclonal IgG1 against a recombinant human Snail protein. Reacts with human, mouse and rat by western blot.

Western blot

Wild-type, Snail1 KO1 and and Snail1 recovered RMG1 cells. Cells were lysed in sodium dodecyl sulfate (SDS) gel sample buffer.

5% non-fat milk in Tris-buffered saline (TBS).

1:1,000 dilution overnight.

Anti-mouse horseradish peroxidase conjugates (Jackson Immunoresearch Laboratories, Inc, West Grove, PA).

Enhanced chemiluminescence (ECL) (PRN 2106, GE Healthcare, Wauwatosa WI).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.