This is a knockout-validated antibody summary, based on the publication "Snail determines the therapeutic response to mTOR kinase inhibitors by transcriptional repression of 4E-BP1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Snail WT or KO HCT116 cells stably expressing 4E-BP1 shRNA or control shRNA were treated with 100 nM INK128 or DMSO as control for 12 h, followed by western blot analysis for the indicated proteins. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Snail

Catalog number: 3879

Summary: Rabbit monoclonal IgG against a recombinant human Snail protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Western blot

WT and Snail KO HCT116 cells. Cells were lysed in NP-40 lysis buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 10% glycerol, protease, and phosphatase inhibitor cocktail).

1:1,000 dilution.