This is a knockout-validated antibody summary, based on the publication "A novel negative regulatory mechanism of Smurf2 in BMP/Smad signaling in bone", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis with the indicated antibodies to evaluate expression levels in rhBMP2-stimulated BMSCs. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Smurf2

Catalog number: 12024

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Pro160 of human Smurf2 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Western blot

Bone marrow stromal cells (BMSCs) from both WT and Smurf2−/− mice.

PhosphoBLOCKER (Cell Biolabs, Inc., San Diego, CA, USA) for 1 h.

1/1,000 dilution overnight at 4 °C.

Anti-rabbit IgG horseradish peroxidase-conjugated antibody (7074, Cell Signaling Technology) for 1 h at room temperature .

Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Little Chalfont, UK), and the membrane was visualized using an MF-ChemiBIS 3.2 imaging system (DNR Bio-Imaging Systems, Ltd., Israel).