Smad4 antibody | knockout validation | Santa Cruz Biotechnology sc-7966

This is a knockout-validated antibody summary, based on the publication "Smad4 represses the generation of memory-precursor effector T cells but is required for the differentiation of central memory T cells", as cited below [1] and "Mutations in SKI in Shprintzen-Goldberg syndrome lead to attenuated TGF-β responses through SKI stabilization" for western blot knockout validation (figure 1b) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Smad4 antibody | knockout validation | Santa Cruz Biotechnology sc-7966 figure 1
Figure 1. From Figure 1 in [1]. Western blot of thymocyte lysates from 6- to 8-week-old Smad4co/co;Lck-Cre mice and control littermates.
Antibody information

Mouse monoclonal

IgG1 kappa

Company: Santa Cruz Biotechnology

Antibody: Smad4

Catalog number: sc-7966

Summary: Mouse monoclonal antibody raised against full-length (residues 1-552) of human Smad4.

Supplier recommended for WB, IP, IF, IHC, flow cytometry and elisa.

Reacts with human, mouse and rat Smad4.

Validation Method

Western blot

Sample

Lysates of T cells from Smad4co/co and Smad4 deleted mice.

Primary incubation

Anti-Smad4 antibody.

Detection

ECL reagent (Amersham).

References
  1. Cao J, Zhang X, Wang Q, Qiu G, Hou C, Wang J, et al. Smad4 represses the generation of memory-precursor effector T cells but is required for the differentiation of central memory T cells. Cell Death Dis. 2015;6:e1984 pubmed publisher
  2. Gori I, George R, Purkiss A, Strohbuecker S, Randall R, Ogrodowicz R, et al. Mutations in SKI in Shprintzen-Goldberg syndrome lead to attenuated TGF-β responses through SKI stabilization. elife. 2021;10: pubmed publisher