This is a knockout-validated antibody summary, based on the publications "RhoA, Rac1, and Cdc42 differentially regulate αSMA and collagen I expression in mesenchymal stem cells", as cited below [1], and "Colon epithelial cell TGFβ signaling modulates the expression of tight junction proteins and barrier function in mice" for (figure 4a,4c) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: Smad4
Catalog number: ab40759
Summary: Rabbit monoclonal IgG against a synthetic peptide within human Smad4 (aa 500 to the C-terminus). Reacts with human, mouse, and rat. Suitable for western blot and immunohistochemistry (paraffin). Unsuitable for flow cytometry, immunocytochemistry/immunofluorescence or immunoprecipitation.
Western blot
WT and Smad4-KO mesenchymal stem cells.
5% milk in TBS, 0.05% Tween (TBS-T) for 60 min.
Overnight at 4 °C.
Corresponding secondary antibodies (Jackson Immunoresearch) for 60 min.
Luminata Western horseradish peroxidase chemiluminescence substrate detection reagent (Millipore) for 1 min at room temperature. Luminescence was detected with medical X-ray film (Agfa).
Representative Western blotting of the indicated proteins in mesenchymal stem cells with knockout of Smad4. Please see Figure 2c in the article [1].
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