This is a knockout-validated antibody summary, based on the publication "Imatinib mesylate inhibits the profibrogenic activity of TGF-beta and prevents bleomycin-mediated lung fibrosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Zymed (Thermo Fisher Scientific)
Antibody: Smad3
Catalog number: 51-1500
Summary: Rabbit polyclonal antibody raised against a synthetic 20 amino acid peptide corresponding to central region of the human Smad3 linker domain. Supplier recommended for flow cytometry, ICC, IHC, WB and elisa. Antigen affinity-purified. Reacts with human Smad3.
Western blot
Lysates of wild-type and Smad3 -/- mouse embryonic fibroblasts.
Anti-Smad3 antibody.
See Figure 3 in [1].
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- Daniels C, Wilkes M, Edens M, Kottom T, Murphy S, Limper A, et al. Imatinib mesylate inhibits the profibrogenic activity of TGF-beta and prevents bleomycin-mediated lung fibrosis. J Clin Invest. 2004;114:1308-16 pubmed
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.
- geneproduct