This is a knockout-validated antibody summary, based on the publication "Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: Smac
Catalog number: ab32023
Summary: Rabbit monoclonal IgG against a synthetic peptide within Human Smac/ Diablo (C terminal). Reacts with human, mouse, and rat. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence (immunocytochemistry), and flow cytometry.
Western blot
Wild-type or SMAC/OMI-/- E1A/ras transformed MEF cells. Cells were lysed in NP-40 lysis buffer (1% NP-40, 1mM EDTA, 150mM NaCl, 50 mM Tris pH 7.4, 1 mM PMSF and complete protease inhibitors (Roche)).
1/1000 dilution overnight in 4°C.
HRP-linked secondary antibodies.
Wild-type or SMAC/OMI-/- E1A/ras transformed MEFs were treated with ABT-737 (10 μM) and UMI-77 (10 μM) and Q-VD-OPh (30 μM) for 4 h. Cell lysates were
immunoblotted for indicated proteins. Please see Figure 4f in the article [1].
The same clone (Y12) is sold as Abcam ab32023, ab199317; EMD Millipore 04-578.
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