This is a knockout-validated antibody summary, based on the publication "Resistance to PARP inhibitors by SLFN11 inactivation can be overcome by ATR inhibition", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Confirmation of SLFN11 deletion in DU145 (A), CCRF-CEM (B), MOLT4 (C) and EW8 cells (D) by western blotting using the indicated antibodies (A-D), and by immunofluorescence using anti-SLFN11 antibody (A and B). Two independent SLFN11-del clones targeted with different sequences by CRISPR/Cas9 system were generated for each cell line SLFN11-del (A) and SLFN11- del (B). From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz Biotechnology

Antibody: Slfn11

Catalog number: sc-374339

Summary: Mouse monoclonal IgG1 (kappa light chain) against an epitope mapping between amino acids 733-765 near the C-terminus of Slfn11 of human origin. Reacts with human by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot | Immunocytochemistry

Wildtype and SLFN11-knockout in DU145, CCRF-CEM, MOLT4 and EW8 cells. WB: whole cell lysates were prepared with the CelLytic™M lysis reagent (C2978, Sigma-Aldrich). IC: cells were fixed for 10 min with freshly prepared 4% (wt/vol) paraformaldehyde in phosphate buffered saline (PBS), and then permeabilized for 10 min in 0.5% Triton-X100/PBS.

IC: 5% bovine serum albumin/0.1% Tween 20/PBS for 1 hour.

IC: for 2 hours.

WB: horseradish peroxidase (HRP)-conjugated antibodies to mouse IgG (GE Healthcare, UK).

IC: Alexa Fluor 488 and/or Alexa Fluor 568 for 1 hour.

IC: confocal microscope (Nikon PCM2000).