Skp2 antibody | knockout validation | Cell Signaling 2652

This is a knockout-validated antibody summary, based on the publication "SKP2- and OTUD1-regulated non-proteolytic ubiquitination of YAP promotes YAP nuclear localization and activity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Skp2 antibody | knockout validation | Cell Signaling 2652 figure 1
Figure 1. Immunoblotting of SKP2 and α-tubulin in three independent SKP2 knockout HEK293A clones generated by CRISPR-Cas9. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Skp2

Catalog number: 2652

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the amino terminus of human Skp2 protein. Reacts with human and monkey. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), and immunofluorescence/immunocytochemistry.

Validation Method

Western blot

Sample

Control and SKP2 knockout HEK293A clones.

Primary incubation

1:1,000 dilution

Secondary incubation

Peroxidase-conjugated secondary antibodies.

Detection

Chemiluminescence (Denville Scientific).

References
  1. Yao F, Zhou Z, Kim J, Hang Q, Xiao Z, Ton B, et al. SKP2- and OTUD1-regulated non-proteolytic ubiquitination of YAP promotes YAP nuclear localization and activity. Nat Commun. 2018;9:2269 pubmed publisher