This is a knockout-validated antibody summary, based on the publication "Sirtuin 7 Deficiency Ameliorates Cisplatin-induced Acute Kidney Injury Through Regulation of the Inflammatory Response", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. SIRT7 expression in mouse kidney, liver, and heart was evaluated. GAPDH and β-actin were used as loading controls. SIRT7 protein expression was deleted in Sirt7 KO mouse kidney.

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: SirT7

Catalog number: 5360

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Figure 2. Representative photomicrographs (×400) of immunohistochemical staining for SIRT7 in kidney sections. Scale bar: 50 µm. From [1].

Summary: Rabbit monoclonal IgG against a recombinant protein specific to the amino terminus of human SirT7 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Western blot | Immunohistochemistry

WT and Sirt7 KO mouse kidney. WB: Total lysates of cells and kidney tissues were obtained by lysis in RIPA Buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1% sodium dodecyl sulfate (SDS), 1% NP-40, 5 mM EDTA, 0.5% sodium deoxycholate, 20 mg/mL Na3VO4, 10 mM NaF, and 1 mM PMSF) with a protease inhibitor cocktail (Nacalai Tesque).

IHC: 1:300 dilution.

IHC: Biotinylated goat anti-rabbit IgG antibody and HRP-labeled streptavidin (Nichirei).

WB: Chemi-Lumi One Super (Nacalai Tesque) and a LAS-1000 imaging system (Fujifilm, Tokyo, Japan).