SirT3 antibody | knockout validation | Cell Signaling 2627

This is a knockout-validated antibody summary, based on the publication "Deacetylation of serine hydroxymethyl-transferase 2 by SIRT3 promotes colorectal carcinogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

SirT3 antibody | knockout validation | Cell Signaling 2627 figure 1
Figure 1. Western blot detection of endogenous SIRT3 in cytosolic and mitochondrial fractions of control and SIRT3-knockout HCT116 cells after NAM treatment.
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: SirT3

Catalog number: 2627

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the sequence of human SirT3 protein. Reacts with human, rat, and monkey. Suitable for western blot. Immunoprecipitation and immunohistochemistry (paraffin).

SirT3 antibody | knockout validation | Cell Signaling 2627 figure 2
Figure 2. Western blot detection of endogenous SIRT3 in WT and Sirt3−/− MEFs. From [1].
Validation Method

Western blot

Sample

Control and SIRT3-knockout HCT116 cells, and WT and Sirt3−/− MEFs.

Primary incubation

1:1,000 dilution.

References
  1. Wei Z, Song J, Wang G, Cui X, Zheng J, Tang Y, et al. Deacetylation of serine hydroxymethyl-transferase 2 by SIRT3 promotes colorectal carcinogenesis. Nat Commun. 2018;9:4468 pubmed publisher