This is a knockout-validated antibody summary, based on the publication "Inhibition of mTOR complex 2 induces GSK3/FBXW7-dependent degradation of sterol regulatory element-binding protein 1 (SREBP1) and suppresses lipogenesis in cancer cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal

Company: Bethyl

Antibody: Sin1

Catalog number: A300-910A

Summary: Rabbit polyclonal against an epitope mapped between residue 470 and C-term of the protein. Reacts with human and mouse. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence and PLA.

Western blot

WT and Sin1-KO MEFs. Lysates were prepared with lysis buffer (50 mM Tris-HCl, pH 8.0, 150 mM sodium chloride, 2.0% Igepal CA-630, 0.5% sodium deoxycholate, 0.2% sodium dodecyl sulfate, 1 mM EDTA and 1% Triton X-100)

Please see Figure 2c in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.