Shank3 antibody | knockout validation | NeuroMab 73-344

This is a knockout-validated antibody summary, based on the publication "Oxytocin improves behavioral and electrophysiological deficits in a novel Shank3-deficient rat", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Shank3 antibody | knockout validation | NeuroMab 73-344 figure 1
Figure 1. Left; Western blot analyses using anti-Shank3 antibodies (targeted against the SH3 domain) and anti-βIII-tubulin in prefrontal cortex postsynaptic density samples in WT and Shank3-heterozygous (Het) and Knockout (KO) rats. From [1].
Antibody information

Mouse monoclonal IgG2a

Company: NeuroMab

Antibody: Shank3

Catalog number: 73-344

Summary: Mouse monoclonal IgG2a against a fusion protein containing a.a. 538-626 (SH3 domain) of rat Shank3. Reacts with mouse and rat. Suitable for western blot, immunocytochemistry and immunohistochemistry.

Validation Method

Western blot

Sample

Brain from WT, Shank3-heterozygous and Shank3-KO rats.

Primary incubation

1:100 dilution.

Secondary incubation

1:5,000 dilution HRP-conjugated anti-mouse antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA).

Detection

ECL substrate (Pierce; Thermo Scientific, Rockford, IL) or SuperSignal West Femto (Thermo Scientific, Rockford, IL) substrates were used to produce the signal that was detected on a G:Box Chemi-XT4 GENESys imager (Syngene; Cambridge, UK).

References
  1. Harony-Nicolas H, Kay M, Hoffmann J, Klein M, Bozdagi-Gunal O, Riad M, et al. Oxytocin improves behavioral and electrophysiological deficits in a novel Shank3-deficient rat. elife. 2017;6: pubmed publisher