This is a knockout-validated antibody summary, based on the publication "Oxytocin improves behavioral and electrophysiological deficits in a novel Shank3-deficient rat", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Left; Western blot analyses using anti-Shank3 antibodies (targeted toward amino acids 840–857) and anti-βIII-tubulin in prefrontal cortex postsynaptic density samples in WT and Shank3-heterozygous (Het) and Knockout (KO) rats. Right; quantification of Shank3 protein levels, based on Western blot analysis of three samples per genotype normalized to βIII-tubulin and then to the WT levels. From [1].

Mouse monoclonal IgG2b

Company: NeuroMab

Antibody: Shank3

Catalog number: 73-109

Summary: Mouse monoclonal IgG2b against a synthetic peptide corresponding to a.a. 840-857 of rat Shank3. Reacts with human, mouse and rat. Suitable for western blot, immunoprecipitation and immunohistochemistry.

Western blot

Brain from WT, Shank3-heterozygous and Shank3-KO rats.

1:100 dilution.

1:5,000 dilution HRP-conjugated anti-mouse antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA).

ECL substrate (Pierce; Thermo Scientific, Rockford, IL) or SuperSignal West Femto (Thermo Scientific, Rockford, IL) substrates were used to produce the signal that was detected on a G:Box Chemi-XT4 GENESys imager (Syngene; Cambridge, UK).