This is a knockout-validated antibody summary, based on the publication "Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Total cell lysates from mouse embryonic fibroblasts (MEFs) with the indicated genotypes were analyzed by immunoblotting. Actin was used as loading control. From [1]

Mouse monoclonal IgG

Company: Santa Cruz Biotechnology

Antibody: Sestrin1, PA26

Catalog number: sc-376170

Summary: a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml; specific for an epitope mapping between amino acids 403-441 near the C-terminus of PA26 of human origin; recommended for detection of all PA26 isoforms of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian

Western blot

Sesn1+/+, Sesn1+/- and Sesn1−/− mouse embryonic fibroblasts. Cells were lysed in 1X Cell Lysis Buffer (CST) supplemented with one tablet of EDTA-free protease inhibitor (Roche) per 50 ml and one tablet of PhosSTOP (Roche) per 10 ml.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.