This is a knockout-validated antibody summary, based on the publication "Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Goat polyclonal IgG

Company: Santa Cruz

Antibody: Sema3A

Catalog number: sc-1148

Summary: Goat polyclonal IgG against an epitope mapping at the N-terminus of SEMA3A of human origin. Recommended for detection of SEMA3A of mouse, rat, human, and chicken origin by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including and equine, canine, bovine, porcine and avian.

Western blot

Brain tissue from Sema3A+/+ and Sema3A-/- mice.

At 4°C overnight or at room temperature for 1 h.

Horseradish peroxidase-conjugated secondary antibody at a 1:5,000 dilution for 1 h at room temperature.

Using Western blotting detection reagents and X-ray film.

Please see Figure 2c in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.