This is a knockout-validated antibody summary, based on the publication "The polarity protein Scrib mediates epidermal development and exerts a tumor suppressive function during skin carcinogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Representative immunofluorescence of Scrib  (green) in Scrib+/+, Scrib +/fl and Scrib fl/fl adult dorsal epidermis (n = 3). DAPI was used for nuclei stain (blue). Scale bar = 50 μm (insert 1–3: scale bar = 10 μm). From [1].

Goat polyclonal IgG.

Company: Santa Cruz Biotechnology

Antibody: Scrib

Catalog number: sc-11049

Summary: Goat polyclonal IgG against an epitope mapping near the C-terminus of Scrib of human origin. Recommended for detection of Scrib of human origin by western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin).

Immunohistochemistry

Epidermis of K14Cre+/−;Scrib+/+, K14Cre+/−;Scrib+/fl and K14Cre+/−;Scribfl/fl adult mice.

1:150 dilution.

Scrib (C-20) will be discontinued December 31, 2016; to receive a 10 µg sample of Scrib (D-2): sc-374139, our highly recommended and much superior monoclonal antibody alternative to this product, please contact Technical Service.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.