This is a knockout-validated antibody summary, based on the publication "Liver kinase b1 is required for white adipose tissue growth and differentiation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Company: Santa Cruz

Antibody: LKB1

Catalog number: sc-32245

Summary: Mouse monoclonal IgG2b raised against recombinant LKB1 of human origin. Recommended for detection of LKB1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin).

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Figure 1. Western blot analysis of LKB1 on WT and LKB1-deficient white adipose tissue, from Figure 4 [1].

Western blot analysis

Fresh fat tissues were forzen in liquid nitrogen before being extracted in lysis buffer with sonication.

The same clone (Ley 37D/G6) is sold as Santa Cruz Biotechnology sc-32245; Abcam ab15095.