This is a knockout-validated antibody summary, based on the publication "FANCI Regulates Recruitment of the FA Core Complex at Sites of DNA Damage Independently of FANCD2", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Company: Santa Cruz

Antibody: FANCI

Catalog number: sc-271316

Summary: Mouse monoclonal IgG1 raised against amino acids 1002-1103 of FANCI protein of human origin. Recommended for detection of human FANCI by western blot, immunoprecipitations, immunofluorescence and ELISA.

Figure 1. Western blot of FANCI protein in soluble (S) and insoluble fractions (I) of wild-type and FANCI-/- HCT116 cells, from [1].

WB, western blot

FANCI-null HCT116 cells were lysed in 0.05 M Tris-HCl (pH 6.8), 2% SDS, 6% β-mercaptoethanol and boiled for 5min.

blocking buffer (5% milk in TBS-Tween 20)

Primary antibodies were diluted in blocking buffer (5% milk in TBS-Tween 20) and incubated overnight.

Horseradish peroxidase—conjugated anti-mouse.

Images were acquired using ImageQuant LAS4000 system (GE Healthcare)

FANCI-null HCT116 cells were generated through recombinant adeno-associated virus-mediated gene targeting.