This is a knockout-validated antibody summary, based on the publications "Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection", as cited below [1], and "Targeting the interaction between RNA-binding protein HuR and FOXQ1 suppresses breast cancer invasion and metastasis" for (figure 1c, s5a,s4) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

HuR, also called ELAV1; HUR; Hua; MelG; ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R); Hu antigen R; embryonic lethal, abnormal vision, drosophila, homolog-like 1; hu-antigen R.

Figure 1. Western blot of different HuR knockout clones obtained by CRISPR from 293-TREx cells. Pum-2 was used as loading control. Courtesy of the authors.

Catalog number is sc-5261.

Antibody (3A2) is a mouse monoclonal IgG1 raised against full length HuR of human origin recommended for detection of HuR, HuB, HuC and HuD of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA.

Santa Cruz Biotechnology.

Western blot

293-TREx cells. Lysed in 10 µl of 1× Passive Lysis Buffer (Promega’s Dual-Luciferase Reporter Assay System, Cat: E1910).

5 % milk in TBST, 1 h at room temperature

1:1000–1:5000 dilution, in 5 % milk in TBST for 1 h, with 3 × 5 min TBST washes in between incubation steps.

Anti-Mouse-HRP-conjugated secondary antibody was from Cell Signaling (anti-mouse, Cat: 7076).

(ECL) Biorad ChemiDoc MP imager

Clone 5.B5 selected and sequenced. HuR deletion confirmed.

The same clone (3A2) is sold as Santa Cruz Biotechnology sc-5261; Invitrogen 39-0600, MA1-167.