This is a knockout-validated antibody summary, based on the publication "An epigenetic switch ensures transposon repression upon dynamic loss of DNA methylation in embryonic stem cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of SUV39H1 protein levels in Suv39h-dKO ES cells. Histone H3 was used as loading control. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: SUV39H1

Catalog number: 8729S

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Asp380 of human SUV39H1 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation. It is predicted to react based on 100% sequence homology with bovine.

Western blot

J1 control and SUV39H1/SUV39H2 double-knockout (Suv39h-dKO) ES cells. Cells were resuspended in BC250 lysis buffer (25 mM Tris pH 7.9, 0.2 mM EDTA, 20% Glycerol, 0.25 M KCl and protease inhibitor cocktail from Roche).

1/1,000 dilution.

HRP-conjugated secondary antibodies.

ECL prime kit (Amersham) and ImageQuant Las-4000 mini biomolecular Imager.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.