This is a knockout-validated antibody summary, based on the publication "Nuclear-cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. From Figure S2 in [1]. Western blot with antibody-SUN2 antibody.

Rabbit monoclonal

Company: Abcam

Antibody: SUN2 (Clone EPR6557)

Catalog number: ab124916

Summary: Rabbit monoclonal antibody raised against a synthetic peptide corresponding to the C-terminal region of human SUN2.

Recombinant rabbit antibody.

Supplier recommended for WB, ICC ad IHC.

Reacts with human, mouse and rat SUN2.

Western blot

Lysates of wild-type and SUN2 knockout primary mouse keratinocytes.

TBS-T containing 10% milk powder.

Anti-SUN2 antibody diluted 1:1000.

HRP-conjugated secondary antibody diluted 1:5000 (ThermoFisher Scientific).

SuperSignal West Femto chemiluminescent reagent (ThermoFisher Scientific).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.