STK38 antibody | knockout validation | Novus Biologicals H00011329-M02

This is a knockout-validated antibody summary, based on the publication "Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis of the expression of NDR1 protein in wildtype and NDR1 knockout mouse primary peritoneal macrophages. From [1].

Antibody information

Mouse monoclonal IgG2a kappa

Company: Novus Biologicals

Antibody: STK38

Catalog number: H00011329-M02

Summary: Mouse monoclonal IgG2a kappa against a partial recombinant fragment of STK38 (NDR1) (365 a.a. ~ 466 a.a). Reacts with human, mouse and rat. Suitable for western blot, immunohistochemistry (paraffin), immunohistochemistry and ELISA.

Validation Method

Western blot

Sample

WT and NDR1 knockout mouse primary peritoneal macrophages.

Primary incubation

1:1,000 dilution.

Clone note

The same clone (3A5) is sold as LifeSpan Biosciences LS-C133688; Abnova H00011329-M02; Sigma-Aldrich SAB1408832.

References

Liu Z, Qin Q, Wu C, Li H, Shou J, Yang Y, et al. Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop. Nat Commun. 2018;9:2789 pubmed publisher

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