STK25 antibody | knockout validation | Abcam ab157188

This is a knockout-validated antibody summary, based on the publication "STK25 suppresses Hippo signaling by regulating SAV1-STRIPAK antagonism", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

STK25 antibody | knockout validation | Abcam ab157188 figure 1
Figure 1. Immunoblots of total lysates of control, and GCKIII (STK25, MST3 and MST4) knockout (KO) 293A cells with the indicated antibodies. Anti-GAPDH blot was used as the loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: STK25

Catalog number: ab157188

Summary: Rabbit monoclonal IgG against a synthetic peptide within human STK25 (aa 1-100, N terminal). Reacts with human. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence. Unsuitable for flow cytometry or immunoprecipitation.

Validation Method

Western blot

Sample

Control STK25 knockout 293A cells.

Detection

Odyssey Infrared Imaging System (LI-COR).

References
  1. Bae S, Ni L, Luo X. STK25 suppresses Hippo signaling by regulating SAV1-STRIPAK antagonism. elife. 2020;9: pubmed publisher