STING antibody | knockout validation | Proteintech 19851-1-AP
DOI
//dx.doi.org/10.13070/ko.en.6.1797
Date
2016-11-08

This is a knockout-validated antibody summary, based on the publication "Activation of STING requires palmitoylation at the Golgi", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

STING antibody | knockout validation | Proteintech 19851-1-AP figure 1
Figure 1. Primary Sting -/- MEFs were mock infected or infected with HSV-1 expressing WT or mutant STING. Probed with anti-STING antibody. From [1].
Antibody information

Company: Proteintech

Antibody: STING

Catalog number: 19851-1-AP

Summary: Rabbit polyclonal antibody raised against a STING fusion protein. Reacts with mouse human and rat. Supplier recommended for elisa, WB, IP, IHC, IF and flow cytometry. Antibody was antigen affinity purified.

Validation Method

Western blot

Sample

Lysates of Sting -/- primary MEFs.

Primary incubation

1:1000 dilution.

Secondary incubation

HRP-conjugated secondary antibody.

Detection

ECL with LAS-4000 (GE Healthcare).

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Mukai K, Konno H, Akiba T, Uemura T, Waguri S, Kobayashi T, et al. Activation of STING requires palmitoylation at the Golgi. Nat Commun. 2016;7:11932 pubmed publisher