This is a knockout-validated antibody summary, based on publications "Intrinsic antiproliferative activity of the innate sensor STING in T lymphocytes" (Figure 1, discussed below) [1], "TRIM29 promotes DNA virus infections by inhibiting innate immune response" (Figure 2) [2], and "Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1β Protein during Virion-Mediated Activation of the AIM2 Inflammasome" (Western blot, Figure 3a) [3], "ZMYND8 Expression in Breast Cancer Cells Blocks T-Lymphocyte Surveillance to Promote Tumor Growth" for western blot knockout validation (figure 3g) [4], "Phosphorylation and chromatin tethering prevent cGAS activation during mitosis" for western blot knockout validation (figure s7c) [5], "MYC suppresses STING-dependent innate immunity by transcriptionally upregulating DNMT1 in triple-negative breast cancer" for western blot knockout validation (figure s6c,s9a) [6], "Reprogramming of nucleotide metabolism by interferon confers dependence on the replication stress response pathway in pancreatic cancer cells" for western blot knockout validation (figure 6c, s4e) [7], "cGAS and DDX41-STING mediated intrinsic immunity spreads intercellularly to promote neuroinflammation in SOD1 ALS model" for western blot knockout validation (figure 3e) [8], and "Docetaxel remodels prostate cancer immune microenvironment and enhances checkpoint inhibitor-based immunotherapy" for western blot knockout validation (figure s5f) [9]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot of STING and actin expression in CD8+ T cells pooled from spleen and lymph nodes of WT and Tmem173gt/gt mice. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: STING

Catalog number: 13647

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Pro226 of human STING protein. Reacts with human and mouse by western blot, immunoprecipitation and immunohistochemistry (paraffin).

Western blot

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Figure 2. Immunoblot analysis of STING in human nasopharyngeal carcinoma CNE1 cells treated with CRISPR vector control or sgRNA targeting mRNA coding region of STING. β-actin serves as a loading control. From [2].

CD8+ T cells (pooled from spleen and lymph nodes) from WT and Tmem173gt/gt (STING-KO) mice. Cells were lysed in sample buffer (4% SDS, 20% glycerol, 0.1 M Tris-HCl, pH 6.8, 0.05% bromophenol blue, and 0.1 M dithiothreitol).

5% BSA (Euromedex) in Tris-buffered saline, 0.1% Tween 20 (AMR ESCO).

Diluted in blocking buffer.

Enhanced chemiluminescence signal was recorded on the ChemiDoc XRS Imager (Bio-Rad Laboratories).