STAT3 antibody | knockout validation | Zymed (Thermo Fisher) 13-7000
DOI
//dx.doi.org/10.13070/ko.en.6.1861
Date
2016-11-25

This is a knockout-validated antibody summary, based on the publication "Malignant transformation but not normal cell growth depends on signal transducer and activator of transcription 3", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal

Company: Zymed (Thermo Fisher Scientific)

Antibody: STAT3

Catalog number: 13-7000 (Clone ST3 5G7)

Summary: Mouse monoclonal antibody raised against a GST fusion protein containing the C-terminal region of mouse STAT3. Supplier recommended for WB, IP, ICC, IHC, IP ChIP, gel-shift and elisa. Reacts with human, mouse and rat STAT3.

Validation Method

Western blot

Sample

Wild-type and STAT3 -/- mouse embryonic fibroblasts.

Primary incubation

Anti-STAT3 antibody.

Figure

See supplemental Figure 1 in [1].

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Schlessinger K, Levy D. Malignant transformation but not normal cell growth depends on signal transducer and activator of transcription 3. Cancer Res. 2005;65:5828-34 pubmed