STAT1 antibody | knockout validation | Santa Cruz sc-592
DOI
//dx.doi.org/10.13070/ko.en.6.1827
Date
2016-11-20

This is a knockout-validated antibody summary, based on the publication "Putting the brakes on mammary tumorigenesis: loss of STAT1 predisposes to intraepithelial neoplasias", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

STAT1 antibody | knockout validation | Santa Cruz sc-592 figure 1
Figure 1. (Immunohistochemistry) Representative pictures of immunohistochemical characterization of two Stat1+/+ (top panel) and two Stat1−/− mammary tumors (bottom panel). Scale bars: 100 μm. (Immunofluorescence) Bright-field (top panel) and fluorescence (bottom panel) microscopy pictures of tumor cell lines derived from two Stat1−/− (#1, #2) and two Stat1+/+ (#3, #4) spontaneous mammary tumors. (Blue) DAPI; (red) STAT1. Scale bars: 50 μm. From [1].
Antibody information

Rabbit polyclonal IgG

Company: Santa Cruz

Antibody: STAT1

Catalog number: sc-592

Summary: Rabbit polyclonal IgG against an epitope mapping at the C-terminus of Stat1 p84/p91 of human origin. Recommended for detection of Stat1β p84 and Stat1α p91 of mouse, rat,and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including equine, canine, bovine and porcine.

Validation Method

Immunohistochemistry | Immunofluorescence

Sample

Mammary tumors and tumor cell lines derived from Stat1+/+ and Stat1−/− mice.

Detection

IF: confocal laser-scanning microscope (Carl Zeiss LSM 700, Occulare 10x, 40x Oil) using the software ZEN 2009 LE.

IHC: Zeiss AxioImager Z1 microscope system with a CCD camera using the software PixelNK Capture 3.0.

Notes

The antibody might be also validated by western blot, authors do not specify.

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Schneckenleithner C, Bago-Horvath Z, Dolznig H, Neugebauer N, Kollmann K, Kolbe T, et al. Putting the brakes on mammary tumorigenesis: loss of STAT1 predisposes to intraepithelial neoplasias. Oncotarget. 2011;2:1043-54 pubmed