This is a knockout-validated antibody summary, based on the publication "Histone chaperone FACT represses retrotransposon MERVL and MERVL-derived cryptic promoters", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of Ssrp1 protein in WT and Ssrp1−/− ESCs. Gapdh was used as a loading control. From [1].

Mouse monoclonal IgG2a κ

Company: Santa Cruz

Antibody: SSRP1

Catalog number: sc-74536

Summary: Mouse monoclonal antibody against amino acids 1-300 of human SSRP1. Recommended for detection of SSRP1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including and canine, bovine and porcine.

Western blot

WT and Ssrp1−/− mouse ESCs.

5% milk.

Anti-mouse IgG-HRP (sc-516102, Santa Cruz).

HRP activity was detected by Luminol HRP Substrate (WBKLS0100, Millipore).