SREBP1 antibody | knockout validation | Novus NB600-582

This is a knockout-validated antibody summary, based on the publication "Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG1 Kappa

Company: Novus

Antibody: SREBP1

Catalog number: NB600-582

Summary: Mouse monoclonal IgG1 Kappa against a fusion protein of human SREBP1 (a.a. 301-407). Reacts with human, mouse, rat, canine, chicken, hamster, primate, and golden Syrian hamster. Suitable for western blot and immunohistochemistry (frozen).

Validation Method

Western blot

Sample
Blocking agent

5% bovine serum albumin.

Primary incubation

1:1,000 dilution.

Secondary incubation

From Cell Signaling Technology (Danvers, MA, USA).

Detection

Millipore enhanced chemiluminescence kit and imaged using ChemiDocTM XRS+ Molecular Imager (Bio-Rad, Hercules, CA, USA).

Figure: The protein level of SREBP1 in WT, OE, and KO Bel-7402 cells. From [1].

Clone note

The same clone (2A4) is sold as Novus Biologicals NB600-582, NBP2-80959; OriGene TA309880; Santa Cruz Biotechnology sc-13551, sc-13551 AC, sc-13551 X; LifeSpan Biosciences LS-C87608, LS-C87609, LS-C143035, LS-C180585, LS-C180586, LS-C180587, LS-C180589; Invitrogen MA5-11685, MA5-16124.

References
  1. Yang N, Li C, Li H, Liu M, Cai X, Cao F, et al. Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells. Front Pharmacol. 2019;10:709 pubmed publisher