This is a knockout-validated antibody summary, based on the publication "Tead and AP1 Coordinate Transcription and Motility", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis of SRC2 in HCT116 cells with or without expression of Crispr knockout constructs against SRC1, SRC2 and SRC3 (SRC1-3 KO). GAPDH was used as loading control. From [1].

Rabbit polyclonal IgG

Company: Bethyl

Antibody: SRC2

Catalog number: A300-346A

Summary: Rabbit polyclonal IgG against an epitope mapping between aa 1400 and C-term of human DOCK9. Reacts with human and mouse protein. Suitable for western blot, immunohistochemistry (paraffin), immunofluorescence, immunoprecipitation and PLA.

Western blot

Control and SRC1-3 KO HCT116 cells.

HRP conjugated secondary antibodies (Jackson Laboratories).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.