This is a knockout-validated antibody summary, based on two publications "Tead and AP1 Coordinate Transcription and Motility" [1] and "Knockout of SRC-1 and SRC-3 in Mice Decreases Cardiomyocyte Proliferation and Causes a Noncompaction Cardiomyopathy Phenotype" [2].. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis of SRC1 in HCT116 cells with or without expression of Crispr knockout constructs against SRC1, SRC2 and SRC3 (SRC1-3 KO). GAPDH was used as loading control. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: SRC1

Catalog number: 2191

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the sequence of human SRC-1 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin) and ChIP.

Western blot

Control and SRC1-3 KO HCT116 cells.

HRP conjugated secondary antibodies (Jackson Laboratories).

Western blot

WT, SRC-1 knockout and SRC-3 knockout hearts at P0, SRC-3f/f, SRC-3d/d, S1KO;SRC-3f/f and S1KO;SRC-3d/d primary cardiomyocytes. Frozen tissue powders and harvested cells were extracted with RIPA buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 0.25% Na-deoxycholate.

Appropriate secondary antibodies conjugated with horseradish peroxidase.

ECL reagent kit.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.