SQSTM1/p62 antibody | knockout validation | Cell Signaling 88588

This is a knockout-validated antibody summary, based on the publication "The role of SQSTM1 (p62) in mitochondrial function and clearance in human cortical neurons", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

SQSTM1/p62 antibody | knockout validation | Cell Signaling 88588 figure 1
Figure 1. Representative fluorescence images of SQSTM1 KO and unedited WT iPSCs (RBi001-A) immunostained against SQSTM1 (green) and Hoechst (blue). Scale bars, 50 mm.
Antibody information

Mouse monoclonal IgG1

Company: Cell Signaling

Antibody: SQSTM1/p62

Catalog number: 88588

Summary: Mouse monoclonal antibody against a synthetic peptide corresponding to residues surrounding Pro220 of human SQSTM1 protein. Reacts with human. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin) and immunofluorescence/immunocytochemistry.

Validation Method

Immunocytochemistry

Sample

WT and SQSTM1-KO iPSCs and differentiated cortical neurons .

Blocking agent

1% bovine serum albumin and 5% normal goat serum in PBST.

SQSTM1/p62 antibody | knockout validation | Cell Signaling 88588 figure 2
Figure 2. Representative fluorescence images of cortical neurons, after 80 days of differentiation, immunostained against SQSTM1 (green), MAP2 (red) and Hoechst (blue). Scale bars, 25 mm. From [1].
Primary incubation

In blocking solution overnight at 4°C.

Secondary incubation

Goat anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific), in blocking solution for 1 h in darkness.

References
  1. Poon A, Saini H, Sethi S, O Sullivan G, Plun Favreau H, Wray S, et al. The role of SQSTM1 (p62) in mitochondrial function and clearance in human cortical neurons. Stem Cell Reports. 2021;16:1276-1289 pubmed publisher