SQSTM1 / p62 antibody | knockout validation | Abcam ab109012

This is a knockout-validated antibody summary, based on the publication "SQSTM1/p62 Promotes Cell Growth and Triggers Autophagy in Papillary Thyroid Cancer by Regulating the AKT/AMPK/mTOR Signaling Pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

SQSTM1 / p62 antibody | knockout validation | Abcam ab109012 figure 1
Figure 1. p62 Knock-out efficiency verified by WB. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: SQSTM1 / p62

Catalog number: ab109012

Summary: Rabbit monoclonal antibody against a synthetic peptide of p62. Reacts with mouse, rat, and human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, and flow cytometry (intra).

Validation Method

Western blot

Sample

WT and p62-KO TPC-1 cells.

Blocking agent

5% skimmed milk.

Primary incubation

1/20,000.

References
  1. Yu F, Ma R, Liu C, Zhang L, Feng K, Wang M, et al. SQSTM1/p62 Promotes Cell Growth and Triggers Autophagy in Papillary Thyroid Cancer by Regulating the AKT/AMPK/mTOR Signaling Pathway. Front Oncol. 2021;11:638701 pubmed publisher