This is a knockout-validated antibody summary, based on the publication "Identification of Enteroendocrine Regulators by Real-Time Single-Cell Differentiation Mapping", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 against
Company: Atlas Antibodies
Antibody: SOX4
Catalog number: AMAb91380
Summary: Mouse monoclonal IgG1 against a synthetic peptide (AGADPSDPLGLYEEEGAG) corresponding to residues of human SOX4. Reacts with human, mouse and rat. Suitable for immunocytochemistry/immunofluorescence and immunohistochemistry.
Immunohistochemistry
Small intestine of wild-type and Sox4 KO mice. Intestines were fixed overnight in formalin, dehydrated using ethanol and embedded in paraffin. 4 mm-sections were processed using standard methods and antigen retrieval was performed using citrate buffer (pH 6.0).
Overnight at 4C.
Confocal microscope.
Immunofluorescent co-staining of NEUROG3 and Sox4 in proximal small intestine of wild-type and Sox4 KO mice. Please see Suppl. Figure 7B in the article [1].
The same clone (CL5665) is sold as Novus Biologicals NBP2-61421; Invitrogen MA5-31424; Atlas Antibodies AMAb91380.
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