SNX5 antibody | knockout validation | Abcam ab180520

This is a knockout-validated antibody summary, based on the publication "Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

SNX5 antibody | knockout validation | Abcam ab180520 figure 1
Figure 1. Western blotting was used to confirm the efficiency of the CRISPR/CAS9 targeting approach of the indicated proteins. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: SNX5

Catalog number: ab180520

Summary: Rabbit monoclonal IgG against a recombinant fragment within human SNX5 (aa 1-150). Reacts with human, mouse and rat. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and immunoprecipitation.

Validation Method

Western blot

Sample

Control and SNX1/2-KO HeLa cells.

Primary incubation

1:1,000 dilution.

Secondary incubation

Fluorescently labeled secondary antibodies.

Detection

LI‐COR Odyssey SA system.

References
  1. Jimenez Orgaz A, Kvainickas A, Nägele H, Denner J, Eimer S, Dengjel J, et al. Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy. EMBO J. 2018;37:235-254 pubmed publisher