This is a knockout-validated antibody summary, based on the publications "A molecular code for endosomal recycling of phosphorylated cargos by the SNX27-retromer complex", as cited below [1] and "Retromer has a selective function in cargo sorting via endosome transport carriers" for western blot knockout validation (figure 5a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1

Company: Abcam

Antibody: SNX27

Catalog number: ab77799

Summary: Mouse monoclonal IgG1 against a fusion protein corresponding to human SNX27 (N terminal). Reacts with human and monkey. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence and flow cytometry.

Western blot

Control and SNX27-KO HeLa cells. Cells were homogenized in buffer containing 20 mM

HEPES, pH 7.4, 250 mM sucrose, 1 mM EDTA, 10 mM Na4P2O7, 30 mM NaF,

2 mM Na3VO4, 0.5 mM AEBSF and protease-inhibitor cocktail with a 22 3/4-gauge

needle.

5% BSA or 5% skim milk in PBS containing 0.1% Tween-20 for 1 h at room temperature.

1:1,000 dilution overnight at 4 °C.

1:4,000 dilution HRP-conjugated goat anti-mouse secondary antibody(62-6520, Life Technologies).

Chemiluminescence signals were detected with a Super Signal ECL detection kit (Thermo Scientific).

Immunoblot analysis of whole cell lysates from control HeLa cells (stably expressing Cas9) and SNX27-KO cells. Na+/K+ ATPase is a control plasma-membrane protein; β-tubulin is a loading control. Please see Figure 7b in the article [1].

The same clone (1C6) is sold as Santa Cruz Biotechnology sc-130564; Abcam ab77799; Invitrogen MA5-27854.