SNX2 antibody | knockout validation | BD Biosciences 611308

This is a knockout-validated antibody summary, based on the publication "Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

SNX2 antibody | knockout validation | BD Biosciences 611308 figure 1
Figure 1. Western blotting was used to confirm the efficiency of the CRISPR/CAS9 targeting approach of the indicated proteins. From [1].
Antibody information

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: SNX2

Catalog number: 611308

Summary: Mouse monoclonal IgG1 against human SNX2 (aa. 15-137). Reacts with human and dog. Suitable for western blot and immunofluorescence.

Validation Method

Western blot

Sample

Control and SNX1/2-KO HeLa cells.

Primary incubation

1:1,000 dilution.

Secondary incubation

Fluorescently labeled secondary antibodies.

Detection

LI‐COR Odyssey SA system.

References
  1. Jimenez Orgaz A, Kvainickas A, Nägele H, Denner J, Eimer S, Dengjel J, et al. Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy. EMBO J. 2018;37:235-254 pubmed publisher