SNAI2 / Slug antibody | knockout validation | Cell Signaling Technology 9585
DOI
//dx.doi.org/10.13070/ko.en.7.1944
Date
2017-01-13

This is a knockout-validated antibody summary, based on the publication "A new role of SNAI2 in postlactational involution of the mammary gland links it to luminal breast cancer development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal

Company: Cell Signaling Technology

Antibody: SNAI2 / Slug

Catalog number: 9585

Summary: Rabbit monoclonal antibody raised against recombinant human SNAI2 / Slug.

Supplier recommended for WB, IP and IF.

Reacts with mouse and human SNAI2 / Slug.

Validation Method

Western blot

Sample

Lysates of breast tissue from wild-type and SNAI2 knockout mice.

Primary incubation

Anti-SNAI2 antibody.

Secondary incubation

HRP-conjugated anti-rabbit IgG (BioRad).

Detection

ECL reagent (ThermoFisher Scientific).

Figure

See Figure 1 in [1].

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Castillo-Lluva S, Hontecillas-Prieto L, Blanco-Gómez A, Del Mar Sáez-Freire M, García-Cenador B, García-Criado J, et al. A new role of SNAI2 in postlactational involution of the mammary gland links it to luminal breast cancer development. Oncogene. 2015;34:4777-90 pubmed publisher