SMURF1 antibody | knockout validation

SMURF1 antibody | knockout validation | Novus H00057154-M01

This is a knockout-validated antibody summary, based on the publication "Non-proteolytic ubiquitin modification of PPARγ by Smurf1 protects the liver from steatosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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SMURF1 antibody | knockout validation | Novus H00057154-M01 figure 1

Figure 1. Western blot showing the increase of the PPARγ protein in livers of BL-Smurf1KO mice. From [1].

Antibody information

Mouse monoclonal IgG2a Kappa

Company: Novus

Antibody: SMURF1

Catalog number: H00057154-M01

Summary: Mouse monoclonal IgG2a Kappa against SMURF1 (165 a.a. ~ 269 a.a) partial recombinant protein. Reacts with human and mouse. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry, immunohistochemistry (paraffin),

immunoprecipitation and ELISA.

Validation Method

Western blot

Sample

WT and BL-Smurf1KO mouse livers.

Clone note

The same clone (1D7) is sold as LifeSpan Biosciences LS-B5471; Abnova H00057154-M01; Biorbyt orb94707; Sigma-Aldrich WH0057154M1.

References

Zhu K, Tang Y, Xu X, Dang H, Tang L, Wang X, et al. Non-proteolytic ubiquitin modification of PPARγ by Smurf1 protects the liver from steatosis. PLoS Biol. 2018;16:e3000091 pubmed publisher

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