This is a knockout-validated antibody summary, based on the publication "Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of Smurf1 expression levels in WT, Smurf1−/− and Smurf1 rescue MEFs. From [1].

Mouse monoclonal IgG2a (kappa)

Company: Abcam

Antibody: SMURF1

Catalog number: ab117552

Summary: Mouse monoclonal IgG2a (kappa) against recombinant human SMURF1 protein. Reacts with mouse and human. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence and ELISA.

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Figure 2. Western blot of Smurf1 expression levels in different tissues from WT and Smuf1-KO mice. Actin serves as loading control. From [1].

Western blot | Immunohistochemistry

WB: WT, Smurf1−/− MEFs and mouse tissues. Cell lysates were prepared in RIPA buffer with protease inhibitor cocktail. IHC: kidney and colon tissues of WT or Smurf1−/− mice.

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Figure 3. Representative immunohistochemical micrographs showing the expression of Smurf1 in the kidney and colon tissues of WT or Smurf1−/− mice. Bars, 50 µm. From [1].

WB: 1:1,000 dilution.

IHC: 1:100 dilution overnight at 4°C

IHC: secondary antibodies (Dako) for 30 min.

WB: Super Signal chemiluminescence kit (Thermo Fisher Scientific).

IHC: BX51 microscope (Olympus), and images were photographed with a 40× objective.

The same clone (1D7) is sold as LifeSpan Biosciences LS-B5471; Abnova H00057154-M01; Abcam ab117552; Sigma-Aldrich WH0057154M1.