This is a knockout-validated antibody summary, based on the publication "Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot of WCEs in ALC1+/+ and ALC1−/− iCAS9 cells transduced with NT sgRNA and SMUG1 sgRNA following 72 h Dox, probed with ALC1 and SMUG1. Ponceau was used as a loading control. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: SMUG1

Catalog number: ab192240

Summary: Rabbit monoclonal antibody against a recombinant fragment of SMUG1 protein. Reacts with human by western blot and immunoprecipitation.

Western blot

Control and SMUG1−/− eHAP cells.

5% skim milk/TBST (TBS/0.1%Tween-20) for 1 h at room temperature.

Overnight at 4°C.

Horseradish peroxidase conjugated (HRP) for 1 h at room temperature.

Clarity or Clarity Max Western ECL Substrate (Bio-Rad).