This is a knockout-validated antibody summary, based on the publications "Efficacy and biodistribution analysis of intracerebroventricular administration of an optimized scAAV9-SMN1 vector in a mouse model of spinal muscular atrophy", as cited below [1], and "Spinal motor neuron loss occurs through a p53-and-p21-independent mechanism in the Smn2B/- mouse model of spinal muscular atrophy" for western blot knockout validation (figure 4c) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of spinal cord samples from WT, Smn1-knockout (KO) and different Smn1-rescued (Ratio 1, Ratio 4 and ICV) mice. Tubulin was used as loading control. From [1].

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: SMN

Catalog number: 610646

Summary: Mouse monoclonal IgG1 against human SMN aa. 14-174. Reacts with human, mouse, rat, and dog. Suitable for western blot, immunohistochemistry-formalin (antigen retrieval required), bioimaging, and immunofluorescence.

Western blot

Tissues were weighed and homogenized in lysis buffer (10 mmol/l Tris-HCl pH7.4, 1 mmol/l EDTA, 1 mmol/l EGTA, 150 mmol/l NaCl, 4 mmol/l sodium pyrophosphate, 100 mmol/l NaF, 2 mmol/l Na3VO4, 0.5% IGEPAL, 1% Triton-X 100 and protease inhibitors (Complete Mini; Roche Diagnostics, France) using MP FastPrep-24 tubes and a Polytron homogenizer (MP Biomedicals, France).

Odyssey blocking buffer (Li-Cor) for 1 hour at room temperature.

1/4,000 dilution in Dulbecco’s phosphate-buffered saline (DPBS)/Odyssey blocking buffer (1/1 dilution) overnight.

1/10,000 dilution IR Dye 680 goat anti-mouse (A21058, Invitrogen) in Dulbecco’s phosphate-buffered saline (DPBS)/Odyssey blocking buffer (1/1 dilution) for 90 minutes at room temperature.

Odyssey Infrared Imager (LI-COR Biotechnology).