This is a knockout-validated antibody summary, based on the publication "Sequential cancer mutations in cultured human intestinal stem cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: SMAD4

Catalog number: sc-7966

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 1-552 representing full length human Smad4. Recommended for detection of Smad4 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry(paraffin), flow cytometry and ELISA.

Western blot

Wildtype and SMAD4KO human organoids. Samples were lysed using RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1% SDS, 0.5% Na-Deoxycholate, 1% NP-40) containing Complete protease inhibitors (Roche).

Please see Figure 2f in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.